This meant that a microscope could not resolve two objects located closer than λ/2NA, where λ is the wavelength of light and NA is the numerical aperture of the imaging lens. This diffraction ...

The size of the Airy disk depends on the wavelength of the light (λ) and the numerical aperture (NA) of the objective lens. The spatial resolution of the microscope is determined by the ability to ...

Combined High Numerical Aperture and Long Working Distance The effectiveness of a microscope depends on its objective lenses ... Brightfield: A popular technique for observing reflected light from a ...

We photopolymerize complex-shaped particles within glass cells consisting of a standard microscope slide and a 170 μm-thick ... An oil-immersion objective with 100× magnification and high numerical ...