The amplicon approach matches samples to known bacterial taxa based on a single sequence: that of the highly conserved gene for bacterial 16S rRNA. Shotgun sequencing instead takes a genome-wide ...

The shotgun approach, typically thought to be the superior method, may substantially underestimate diversity in environments that have not already been classified, researchers find. The measles virus ...

A Sanger sequencing reaction is run with a single primer ... keeping in mind the maximum is always 1μg. For amplicons you may use the “divide by 50 rule” where you divide the base pair size of the ...

Gain precise, in-depth insights into microbial genomes with Illumina’s comprehensive Methods for Microbial Single-Genome Sequencing guide.

Our researchers are developing fast and portable virus sequencing techniques to track viruses in areas with limited medical ...

transcriptome sequencing, small RNA analysis, and amplicon sequencing. The Genome Sequencer FLX is built upon 454 Sequencing™ technology that allows 200–300 base-pair read lengths and very ...

The previous generation of pan-cancer assays used amplicon-based enrichment. Amplicon sequencing and hybrid capture are both highly specific and sensitive, and can enrich small amounts of nucleic ...

This enzymatic clipping of PCR primers avoids wasted sequencing output on non-informative bases that do not contain genomic inserts. If you wish to sequence the genomic loci contained within a PCR ...

researchers conducted large-scale metagenomic and 16S rRNA gene amplicon sequencing to investigate microbial diversity and adaptation strategies in the hadal zone. During the TS21 expedition ...

and the Illumina MiSeq has a range of outputs and read lengths generally suited to the sequencing of small genomes, amplicons and targeted applications. Both instruments offer a rapid run time ...