Hunter adds, “Primer design algorithm(s) used must account for sequence specificity and limit both off-target and primer dimer effects and the DNA polymerase used for amplification must be ...

PCR Enhancement Products Courtesy of RedasoftRedasoft's Visual Cloning 2000 includes primer design tools. Courtesy of Sigma-AldrichDirect loading of PCR products onto an agarose gel using ...

This allows the supernatant containing smaller DNA fragments, such as primers and primer dimers, to be easily aspirated and discarded, separating the nucleic acids. Step 2: Washing.