New techniques in quantitative real-time PCR offer enhanced possibilities for the diagnosis of bacterial diseases, while facilitating rapid assessment of antibiotic resistance in bacterial species.

In plants there are three microtubule arrays involved in cellular morphogenesis that have no equivalent in animal cells. In animals, microtubules are decorated by another class of proteins – the ...

Real-time PCR Technology Although extraction of bacterial nucleic acids from clinical samples remains of variable sensitivity, either using manual or automated methods, currently available methods ...

Each 100 μl reaction contained 2.5 U of Pfu Turbo polymerase (Stratagene), 1× cloned Pfu buffer (10 mM (NH 4) 2 SO 4, 20 mM Tris pH 8.8, 2 mM MgSO 4, 10 mM KCl, 0.1% Triton X-100, and 0.1 mg ml ...