First strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (RNase H), followed by second strand cDNA Synthesis using DNA Polymerase I and RNase H.

First strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (RNase H), followed by second strand cDNA Synthesis using DNA Polymerase I and RNase H.

Cello, J., Paul, A. V., & Wimmer, E. (2002). Chemical Synthesis of Poliovirus cDNA Generation of Infectious Virus in the Absence of Natural Template. Science, 297 ...

Synthesis of therapeutically relevant blockmers (5-6-nt) The ezRNA™ platform showcased clear differentiation from templated enzymatic synthesis platforms and its ability to complement RNA ...

For DNA to be copied, the DNA double helix must open up, and the resulting single strands—each of which serves as a template for building a new, second strand—are vulnerable to breakage.

First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then RNaseH was used to degrade the RNA. Second strand cDNA synthesis was subsequently performed using ...

Second strand cDNA synthesis was subsequently performed using buffer, dNTPs (dUTP), DNA polymerase I and RNase H. RNA fragmentation and short RNA strands were carried out by NEBNext First Strand ...

Abstract Transcriptome profiling by RNA sequencing (RNA-seq) has been widely used to characterize cellular status, but it relies on second-strand complementary DNA (cDNA) synthesis to generate initial ...